|
KMID : 0380620140460060729
|
|
Korean Journal of Food Science and Technology
2014 Volume.46 No. 6 p.729 ~ p.733
|
|
|
Anti-inflammatory Activity of an Ethanol Extract of Laminaria japonica Root on Lipopolysaccharide-induced Inflammatory Responses in RAW 264.7 Cells
|
|
Kang Bo-Kyeong
Kim Koth-Bong-Woo-Ri
Kim Min-Ji
Bark Si-Woo
Pak Won-Min
Kim Bo-Ram
Ahn Na-Kyung
Choi Yeon-Uk
Ahn Dong-Hyun
|
|
|
Abstract
|
|
|
|
Laminaria japonica roots have not been used practically in Korea. In this study, in order to promote the use of these by-products, the anti-inflammatory activity of an ethanol extract of Laminaria japonica root (LJREE) was investigated using a lipopolysaccharide (LPS) to induce an inflammatory response in RAW 264.7 cells. To examine the potential anti-inflammatory effects of LJREE, levels of nitric oxide (NO) and pro-inflammatory cytokines, such as interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β), and cell proliferation were measured. We found that NO levels decreased in a dose-dependent manner, the production of IL-6, TNF-α, and IL-1β was suppressed, and the production of IL-1β was inhibited over 30% after treatment with 100 μg/mL LJREE. In conclusion, the proliferation of RAW 264.7 cells, measured by MTT assay, confirmed that LJREE may have significant effects on inflammatory factors without any cytotoxicity, making it a potential anti-inflammatory agent.
|
|
|
KEYWORD
|
|
|
Laminaria japonica root, anti-inflammatory effect
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|